In our previous study , yggg , speb were found in 2 . 8kb dna fragment that could bound era probe by using e . coli genome phage expression library to screen era binding protein 根据本课题组筛选era结合蛋白时获得的噬菌体中的插段包含大肠杆菌的ygggspeb基因,本研究分析了era与ygggspeb的相互作用。
Tanaka reported a novel method to isolate genes of drug - binding proteins directly from a cdna expression library by using the combination of drug and marker molecule 即利用标记分子与药物结合作为探针,直接从cdna表达文库筛选药物结合蛋白的基因并分离和体外表达,以便获取目的蛋白进行结构和功能研究。
So , the identification and characterization of its binding proteins will help to explore the physiological functions involved . to accomplish this goal , different methods were employed in this study 研究表明prpc发挥正常功能需要与另一蛋白结合协助作用,因此对其受体的分离和鉴定也有助于进一步探索其生理功能。
Cortactin , a novel member of filament actin binding protein family and the main substrate of non - receptor src protein kinase , plays an important role in the dynamic organization of cell cortex cytoskeleton 皮层蛋白( cortactin )是一种微丝肌动蛋白结合蛋白,它与肌动蛋白纤丝的侧面相结合,并直接参与皮层细胞骨架的组建。
Analysis of copper binding protein by sds - page , three main protein bands observed . the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc . 4 Sds page分析得到3条主要蛋白带,剪下这三条带进行胶内赖氨酸内切酶的消化,通过高效液相色谱分离肽段,选择性进行肽段的氨基酸序列测定。
Erebps ( ethylene - responsive element binding proteins ) belong to a family of transcription factors , which regulate ethylene responses through binding to the promoter region of ethylene - regulated target genes Erebps (乙烯反应元件结合蛋白)是一个转录因子家族,通过与乙烯调节的目标基因的启动子区结合而调节目标基因的表达。
The greatest progress has been made using genetic , biochemical and cell biological approaches to study aba binding proteins , however , no aba putative receptors have been identified to date 尽管,通过遗传学、生物化学、细胞生物学方法, aba结合蛋白的研究取得了巨大的进展。但是,遗憾的是至今仍然还未鉴定出任何可能的aba受体。
Recently , two distinct genes , dreb1 and dreb2 , encoding dna binding proteins that specifically bind to the dre sequence of rd29a gene had been cloned from arabidopsis by using the one - hybrid screening technique 它们编码着与拟南芥rd29a基因的dre顺式作用元件特异结合的转录因子,参与在干旱,高盐及低温胁迫条件下的rd29a基因的表达调控作用。
Grp94 functions as molecular chaperones , which are ( poly ) peptide binding proteins that assist in protein folding , assembly and transport . moreover , its role of the transporter for antigen presentation has been discovered 目前已知grp94的功能主要是作为分子伴侣,在新生肽链合成的早期阶段,与新生肽键形成稳定的复合物,参与蛋白质的折叠、装配和转运。
Talin is a important f - actin binding protein , two functions of intact talin are the control of actin filament length and the cross - linking of actin polymers . the binding of f - actin to talin significantly increases actin filament stiffness Talin是一个重要的f - actin结合蛋白,它可以控制微丝的长度,并且同actin聚合体发生交联, f - actin和talin发生的结合可以显著地增加微丝骨架的强度。