go down to posterity; go down to the future generation; hand from generation to generation; passage ◇传代时间 generation [transfer] time; 传代网 plexus of descent
( l ) . cultivation of dermal fibroblast : dermal fibroblasts were harvested by using enzyme digest technique from child foreskin after circumcision . 3 - 4 generation of cells were used as work cells . ( 2 ) cultivation of dermal fibroblast on collagen - chitosan spongy membrane 真皮成纤维细胞的分离培养:取包皮环切术后的幼儿包皮酶消化法分离培养真皮成纤维细胞并传代,以3 4代细胞作为工作细胞。
But we got few colonies when pgcs were cultured on sto extracellular matrix or on 0 . 1 % gelatin even in brl - cm . ( 8 ) with the support of sto feeders , addition of growth factors is not reqired for survival and proliferation of curtured porcine pgcs ( p > 0 . 05 ) ( 8 )以sto细胞为饲养层,培养液中加否生长因子对eg培养效果影响不大( p 0 . 05 ) ,但培养液中加入生长因子后,对eg细胞培养传代更有利。