go down to posterity; go down to the future generation; hand from generation to generation; passage ◇传代时间 generation [transfer] time; 传代网 plexus of descent
11 pgcs were isolated from 55d goat and cloned to seconed passage , nor pgcs from 70th day goat embryonic genital . the presence of lif had no positive influence on the isolation of es - like cell 55d的胎儿获得11个pgcs集落,可传代2次,从70d胚胎的生殖腺没有分离到pgcs细胞集落。
2n methods 1 ) sample preparation the supernatant of cultures were digested with identical volume lysis buffer at 100 for 15 min . dna was isolated for use as template 二、实验方法1 、标本处理取hcmv低传代分离株细胞培养上清液与等量裂解液混匀后,煮沸15分钟,提取dna ,作为扩增模板。
The transformants were cultivated and the harvested cells were used in the procedure of conjugation by which the recombinant plasmid pid03 and pc05 were introduced into streptomyces avermitilis s - 2 respectively 经过在mym平板上的传代和抗性标记的筛选分别得到了同源双交换的菌株aved24和avecg 。
The results of both of virulent attack test and 2 - de were paralleled in the following generations , with decrease of virulence of aeromonas hamphila in the former , and deletion of toxin - related genes in latter 攻毒实验和2 - de的结果均表明在传代过程中一些毒力相关的因子随着传代的进行而逐渐消失。
The effect of the three culture systems on formation of es colonies at primary passage was not remarkable but significant in . es colonies formation after passage and the highest times of passage 试验中设计的三种培养条件对原代es集落的形成影响不显著,但对传代后的es集落的形成和传代的代次有显著差异。
During the course of the primary culture and passage of es cells , co - culture of mouse es cells and mef was the best . in this research , cell digestive juice with 0 . 125 % trypsin + 0 . 02 % edta was relative gentle to cells 消化液浓度以0 . 125胰酶+ 0 . 02 edta对es细胞的损伤力最小,传代后es克隆出现率最高。
Continuous passage of neurospheres was used to isolate , culture nscs from dentate gyrus of hippocampus of newborn rats and to observe the self - renewal of neurospheres , which were immunostained with nestin 采用神经球连续形成法从新生大鼠海马齿状回分离、培养神经干细胞,通过连续传代培养观察其自我更新能力。
1 . 1 observation of culture cells with phase contrast microscope the smg cells cultured are polygon epithelium cells and could go down to 3 to 4generations and live up to 3 - 4w in the medium ( dme 1 .相差倒置显微镜下观察smg细胞生长期间呈扁平状,单层生长,为多角形的上皮细胞,细胞可传代生长3一4代,存活3一4w 。
Bovine viral diarrhea virus were propagated by mdbk cells . after five to six continuously and rapidly passing , the cells emerged evident cytopathic effect . then the infected cells and its solution were harvested Bvdvnadl株属于致细胞病变型毒株,用mdbk细胞培养增殖bvdv ,经5 - 6次连续传代至出现明显的细胞病变。
The spores of a . niger m1 , the initial strain , was treated by u . v . at morality of 90 % . a mutant with high xylanase activity was screened , naming a . niger j 506 用紫外线照射的方法对出发菌株aspergillusnigerm1进行处理后,从中筛选到了一株木聚糖酶活力比较高的突变株a . nigerj506 ,传代10次后产酶能力仍然稳定性良好。