结合 1.(发生密切联系; 联合) combine; unite; integrate; link; binding; coalition; cohesion; connection; joint; union; association; join; incorporation; coalescence; coupling; junctura (pl. juncturae); concrescence; nexus; valence; merging; consolidation 与群众相结合 integrate oneself with the masses; become one with the masses; 把理论与实践结合起来 combine [link] theory with practice; 梦游者能把睡眠和运动巧妙地结合在一起。 a somnambulist can happily combine sleep and exercise. 我把你的建议和我的计划结合在一起。 i integrated your suggestion with my plan.2.(结为夫妻) marry; be united in wedlock; be tied in wedlock 他俩的结合违背了各自父母的愿望。 they got married to each other against their parents' wishes.; 结合部位 binding site; 结合层 [土] binder course; close binder; 结合点 binding site; bonding point; 结合方案 association schemes; 结合方式 structure; 结合杆 connecting rod; union link; 结合环 associative rings; 结合剂 agglutinant; binding agent; 结合角钢 connecting angle; 结合力 bond; 结合律 [数学] associative law; law of association; 结合面 [工业] faying face; faying surface; 结合器 colligator; connector; 结合强度 bond strength; 结合体 arrangement; amalgam; [生态学] association; ecological association; 结合性能 binding ability; 结合作用 combination; conjugation
Substrate binding , catalysis , and product release 底物结合催化和产物释放
Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate 连接酶:可将两个底物结合在一起的酶,此过程需要atp或其他核苷三磷酸供能。
A competitive inhibitor is usually structurally similar to the substrate , e . g . malonate is a competitive inhibitor of the enzyme succinate dehydrogenase 与底物结构相似的物质能竞争性的与酶结合,从而使酶不能与底物结合而丧失其催化活性。
Then , a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer . combined with the opuaa - up , a 2 . 3 kb fragment was obtained through pcr . blast result showed a fragment which contained the partial opuaa , the whole opuab and partial opuac sequences were obtained 再次,根据甘氨酸甜菜碱atp转运系统底物结合蛋白的氨基酸保守序列设计下游简并引物,与atp结合蛋白的上游简并引物组合,经pcr扩增获得2 . 1kb的条带,测序后通过blast比较,结果显示获得atp结合蛋白基因的部分序列、通透酶的全部编码序列和部分甘氨酸甜菜碱结合蛋白基因的核苷酸序列。