The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible 随着基因打靶技术和动物克隆技术技术的发展,外源基因在高等哺乳动物基因组中的定点整合成为了可能。
Compared with nucleic genome , the chloroplast genome is very small and easily to be manipulated genetically , and the foreign gene is site - direct integrated into the chloroplast genome through homologous recombination 与核转化相比,叶绿体基因组小,遗传操作简单,外源基因是通过同源重组机制定点整合进叶绿体基因组。
To determine that the integration of foreign genes had occurred hi the chloroplast genome at the directed site by homologous recombiantion and to evaluate the degree of homogeneity of the c . reinhardtii transformants , pcr and pcr - southern blot analysis were performed using the primers of c . reinhardtii chloroplast homologous fragment 并根据衣藻叶绿体同源片段chll基因,设计一对引物对其叶绿体dna进行的pcr和southernblot分析。结果表明外源基因已经定点整合入衣藻叶绿体基因组的chll基因中,并且经过三轮抗生素筛选所得的衣藻叶绿体转化子中,转基因型叶绿体基因组拷贝占多数。