To optimize renal substitution therapy , a bioartificial renal tubule assist device ( rad ) was developed , which is composed of renal proximal tubule cells grown within a hollow fiber cartridge 生物人工肾小管辅助装置( bioartificialrenaltubuleassistdevice , rad )就是利用“组织工程学”原理,通过生物学与工程学的结合构建出的能模拟正常肾小管生理功能的一种人工器官装置。
It is well known that in patients who suffer from starvation , chronic metabolic acidosis , chronic renal insufficiency , distal renal tubular acidosis and nephrolithiasis , the reabsorption of urinary citrate is increased and the excretion is decreased in kidney 研究表明,在饥饿、代谢性酸中毒、肾功能不全、远端肾小管酸中毒及肾结石患者中,肾脏对尿枸橼酸的重吸收增加,尿枸橼酸排泄减少。
Whereas this therapy replaces the partial solute clearance function of the glomerulus but does not replace the transport , and metabolic and endocrinologic activities of the kidney , which are located predominantly in the tubular elements of the kidney 但是当前的血透和血滤技术并不是一种完整的肾脏替代治疗,它们仅仅代替了肾小球的滤过功能,而不能替代肾小管的物质转运、代谢和内分泌等功能。
The endocytosed albumin appears to undergo transcytosis in large esicles ( 500 nm in diameter ) , identified by immunogold staining of endogenous albumin by electron microscopy , to the basolateral membrane where the albumin is disgorged back to the peritubular blood supply 对内源性白蛋白进行免疫金染色然后在电镜下观察发现:这些重吸收的白蛋白以大的囊泡(直径500纳米)形式被跨细胞转运到肾小管基底膜侧,然后回吐到肾小管周血管。
The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days . the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying . the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage . the chicken embryo dead in a week after it inoculated . the body was dropsy and hemorrhage . dead before it hatched out , the embyo body were dropsy , pale and slime . the liver was yellow and swolled , gallbladder ( vesica fellea ) was filled with bile . bursa and glandula thymus analosis . the kindey dropsy . bowel lamina were humble , dilatation . gas and yellow foam were filled the bowel . histopathological changes were that , in early stage , obvious changes of liver and kindey were dropsy , hemorrhage and necrosis . two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs . the obvious changes of bursa were dropsy , adverse folliiculated growth and little lymphocytes proliferating , 19 - 21 days chicken embryo , one or two big empty vacuoles were prensent in cells of liver and kindey . the number of the folliculi was growing , the vacuoles between cells were larger 胆囊充盈、其内充满稀薄的胆汁;法氏囊、胸腺萎缩,肠道扩张、肠壁菲薄、内充满气体及黄色泡沫状物;肾脏肿大。病理组织学变化方面,早期肝脏、肾脏、肠主要以出血、水肿和坏死为主,且肝细胞核及肾小管的上皮细胞核内均发现有核内包涵体,包涵体呈嗜酸性,为大型圆形包涵体或不规则的颗粒状;法氏囊则以水肿、滤泡发育不良、小型淋巴细胞数量增多为主。 19 21日龄鸡胚肝细胞、肾小管上皮细胞的胞浆内出现1 2各大的空泡,法氏囊滤泡数目增多细胞间有较大空隙。