Two types of repeat sequence , a 15 - amino - acid ( eelcaqlcstppppi ) with 2 repeats and a 6 - amino - acid ( ppictp ) with 4 repeats , were firstly reported . 2 . the characterization of meq gene product and its expression within the cells a recombinant baculovirus transfer vector pblubac4 - meq was constructed by cloning meq gene of marek ' s disease virus ( mdv ) ga strain into the baculovirus transfer vector pbluebac4 under the polyhedrin promoter 此外,研究还发现了meq基因的两类有趣的重复结构,其中一类是含15个氨基酸残基( eelcaqlcstppppi )的结构,有2个重复,另一类是含6个氨基酸残基( ppictp )的结构,共有4个重复,它们全部分布在meq蛋白c -端的转录激活域内。
2 . the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein , encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids . 19 highly conserved , potential glycosylation sites and 17 cysteines residues were characterized with si protein , homology analysis showe that there were gene deletion - S1基因:其全序列共1614bp (从起始密码子atg到s前体蛋白裂解位点) ,编码537个氨基酸,其氨基端有一编码18个氨基酸的信号肽序列,第12 13位氨基酸残基构成了信号肽的切割位点, 14 19位与111 124位氨基酸残基为s1蛋白的跨膜区域。
61 of these clones have hybridization signals on the x - photos of the reverse northern blot . then northern blots were performed for 23 of these 61 clones : nos . 4 , 17 , 18 , 23 , 28 , 66 - 1 , 69 , 84 , 89 - 1 , 94 , 97 , 101 , 108 , 152 , 156 - 1 , 157 - 1 , 165 , 172 , 175 , 183 , 185 , 191 and 233 其结构功能域中包含1个28个氨基酸残基的信号肽ol2个晚期胚胎发生高丰度蛋白lea毛结构域( 56 206 , 205 323 ) 、 2个保守分泌蛋白cog5608结构域( 72 200 , 174 281 )和3个低复杂度区门, 4265 , 329习44其h级结构中存在有大量的卷曲螺旋区。
The pocket accommodating the aromatic ring of phenylalanine is formed predominantly by hydrophobic side - chains , including those of ile10 , ile13 , prol50 , leul75 , leul79 , phe209 , ser211 and val221 ; whilst the amino group and carboxylate group of phenylalanine is coordinated by asp6 , asp7 , gln151 and ser180 . the n - terminal region is found to be important to the function of arog . point mutation reveals that residue substitution of ilelo to ala10 leads to desensitization in the presence of 1 mm phenylalanine , simultaneously , exhibits a dramatic decrease in enzymatic activity 综合前人的研究和本研究工作的实验数据,得出以下结论: 1 )在arog的反馈抑制位点供苯丙氨酸结合的疏水口袋由asp6 、 asp7 、 ile10 、 ile13 、 pro150 、 gln151 、 leu175 、 leu179 、 ser180 、 phe209 、 ser211和val221等12个氨基酸残基构成; 2 )苯丙氨酸结合的疏水口袋分为两个区域,与苯丙氨酸的苯环发生疏水作用的区域由ile10 、 ile13 、 pro150 、 leu175 、 leu179 、 phe209和val221的侧链构成,而asp6 、 asp7 、 gln151和ser180的侧链构成了与苯丙氨酸的氨基和羧基相互作用的区域。