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binding site中文是什么意思

  • 接合点
  • 结合部位,结合位点
  • 束缚位置

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  • 例句与用法
  • A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein . the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis . it was demonstrated that constructed phagemid can still express penicillin g acylase
    将包含信号肽和琥珀终止密码子uag ( amber )的完整巨大芽孢杆菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ,通过引入的11肽连接青霉素g酰化酶的c末端与噬菌体外壳蛋白gp3的n末端。
  • Extension of low k + treatment to 12 hours did not increase the binding sites or protein abundance either . among ros species , h2o2 was specifically involved in the up - regulation of na , k - atpase induced by low k . 96 base pairs of upstream of na , k - atpase subunit promoter was the key cis - element in transcriptional regulation of promoter activity
    Ros对钠钾atp酶亚基启动子的刺激作用缩小至编码区上游96碱基对,该序列包含多个潜在sp1转录因子结合位点,研究也表明sp1参与了低钾对钠钾atp酶的调节作用。
  • The latter plays a very important role in the study of the mechanism and dynamics of the recognition . it includes the molecular modeling , confirming the binding sites , the calculation of interactions between receptor and ligand , the docking research of the complex , the calculation of dynamic and thermodynamic properties etc . many theoretical calculation methods are involved , such as quantum chemistry , molecular mechanisms , molecular dynamics , monte carlo method and free energy calculation and so on
    其中计算机模拟方法是研究分子识别机制及其动态过程的重要途径,包括底物及受体分子模型的构建、底物及受体相互作用位点的确定、相互作用力的计算、底物及受体分子的对接及其动态过程的研究、体系热力学及动力学性质的计算等方面内容。
  • Sds - page proved that the molecular weight of expressed product of the extracellular part of bt - r3 was about 160kda . western blotting showed that the recombinant protein could specifically bind to bt toxic protein crylab , indicating that the bt - r3 is a receptor protein of crylab . the binding site of bt - r3 with crylab was located in extracellular domains of bt - r3 protein
    用大肠杆菌系统表达的bt - r3基因的胞外结构域的分子量约为160kda , western - blot实验证实,重组蛋白能有效地结合bt毒蛋白cry1ab ,从而进一步确证了bt - r3是cry1ab的受体基因。
  • Abstract : the effects of terbium ion on the conformation of calmodulin and on the interaction between calmodulin and melittin have been studied by the endogenous fluorescent spectrometry of calmodulin and melittin , and the sensitized fluorescent spectrometry of terbium ion , respectively . the results show that terbium ions have a tight binding site in the i and ii metal - binding sites of calmodulin . the conformation of calmodulin induced by terbium ion can bind melittin and transfer the tryptophane residue of melittin to a relatively hydrophobic environment , while the binding of melittin to calmodulin produces effect on the binding orders of terbium ion in camodulin . results from ft - ir spectrometry have revealed that upon binding of lanthanum ion , apo - calmodulin undergoes a conformational change with the increase of - helix content and the decrease of - sheet content . melittin ' s binding to calmodulin has no effect on its conformation induced by the binding of lanthanum ion to calmodulin
    文摘:分别用钙调蛋白和蜂毒素的内源荧光光谱以及铽离子的敏化荧光光谱考察了铽离子对钙调蛋白构象变化以及对钙调蛋白与蜂毒素相互作用的影响.结果表明,铽离子首先结合在钙调蛋白的第和第位点,铽离子不影响钙调蛋白与蜂毒素的相互作用,蜂毒素与钙调蛋白作用后不影响铽离子在钙调蛋白上的键合顺序.傅里叶变换红外光谱结果表明三价的镧离子与钙调蛋白作用使钙调蛋白的螺旋结构增加,折叠结构减少,与钙离子对它的二级结构影响相类似.稀土离子在钙调蛋白-蜂毒素复合体系中主要与钙调蛋白作用
  • The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry . the su of retroviruses is a highly variable genetic element , containing receptor binding sites and major antigenic determinants . exjsrv - specific dna probes were derived . by using these dna probes in tissue hybridization . we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals , validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
    用地高辛随机引物法标记exjsrv特异的env片段,制备探针,原位杂交检测spa肺组织中的rna及前病毒dna ,结果表明spa患羊肺组织内有jsrvenv基因mrna的表达,同时也检测到了前病毒dna ,而相应的阴性对照却无阳性信号,证实外源性病毒特异的dna探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。
  • The result indicates that the mg2 + ion around the binding site is very important to stabilize the binding of the ligand to the receptor . the binding free energy estimated by the empirical function favors the system with one mg2 + ion , in which the free energy is about 5 kcal / mol less than the system with mg2 + ion
    含有mg2 +离子的情况下比不含mg2 +离子的体系之间的自北京工业大学工学硕士论文一由能之差为一5kcal m ,结果说明结合位点处的mg ‘ ”离子对稳定配体与受体的结合起了重要作用。
  • Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin . the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5 . 4 ) . the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa . a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5 . 4 ) , which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ) . the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed . the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching
    第一部分:等离子点ph ( 5 . 4 )条件下,用平衡透析法和紫外光谱,荧光光谱,共振散射光谱研究了ag ( )与人血清白蛋白( humanserumalbumin ,简称hsa )或牛血清白蛋白( bovineserumalbumin ,简称bsa )的结合。 scatchard图分析表明, ag ( )在hsa或bsa中有强弱两类结合部位,通过计算机拟合获得结合的逐级稳定常数值。紫外扫描发现ag ( )与hsa或bsa的结合存在滞后效应,表明ag ( )与hsa或bsa的结合可能诱导蛋白质构象发生缓慢变化( a - b ) ,测得并讨论了这一构象变化的速度常数和活化参数。
  • By observing the effect of acidity on the uv difference spectra of ehpg or hbed , the spectral characteristics of apoovotransferrin binding to metals and anions at ph 7 . 4 show that the reaction of apoovotransferrin with metals is related to deprotonation of the phenolic group of tyrosine of the metal binding sites , while the binding of apoovotransferrin to anions is associated with the protonation of the phenolic group of tyrosine of the anion binding sites
    摘要通过观察酸度对模拟转铁蛋白小分子ehpg和hbed紫外差光谱的影响,结合脱铁伴清蛋白与金属离子、阴离子结合的光谱特性,推断脱铁伴清蛋白与金属离子的结合导致金属离子结合位点处酪氨酸酚羟基的去质子化,而与阴离子的结合导致阴离子结合位点处酪氨酸酚羟基的质子化。
  • Kyot2 lacked two lim domains from the c terminus and had a frameshift in the last exon , creating the rbp - j - binding region in the c terminus . kyotl had a negligible level of interaction with rbp - j . the binding site of kyot2 on rbp - j overlaps those of ramic and kyot2 repressed the rbp - j mediated transcriptional activation by ramic by competing with them for binding to rbp - j
    在肌肉母细胞系czc12内过表达kyoth时可阻断转录激活物notch胞内区或ebnaz对rbp依赖性启动子的转录激活作用,这种阻断作用呈剂量依赖性,提示kyoth可与notch胞内区或ebnaz竞争rbp j上的结合位点,从而抑制二者的转录激活活性
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  • 百科解释
In biochemistry, a binding site is a region on a protein, DNA, or RNA to which specific other molecules and ions?in this context collectively called ligands?form a chemical bond.
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  • 其他语种释义
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Last modified time:Sun, 10 Aug 2025 00:29:56 GMT

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