The expression of ha in vero cells infected with rprv - ha was detected by western - blot . the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha . the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza 结果表明所获得的重组病毒( rprvha )遗传性状稳定,在培养细胞中能稳定的表达与sivha具有相似生物学活性的外源蛋白,为进一步制备抗猪流感的重组伪狂犬病毒活载体疫苗奠定了基础。
Recently , great progress has been made in understanding of the mechanism of cell movement in vitro , and probably , cortactin acts directly on the molecular machines of cell motility . however , in vivo experimental evidence is still in great need because of tissue untransparancy and deficiency of normal moving cells in adult tissues 近几年来,国际上对于细胞运动分子机制的研究取得很大进展,利用组织培养细胞进行的大量的体外实验证明,皮层蛋白很可能直接参于细胞运动的产生过程。
The results showed that : when cultured in the medium of m199 , supplemented with 20 % bovine serum containing a moderate amount of antibiotics , the incubtion ph 7 . 2 - 7 . 4 , the culture temperature 28 . the primary culture cells formed the dense single wall within three weeks , the generation time of the subculture cells was 5 - 6 days , most cultured cells were fibroblastic - like cells with few epithelial - like cells 研究初步表明:在m199培养基加入20小牛血清(常规量双抗) , ph在7 . 2 - 7 . 4之间, 28的培养条件,四倍体鲫鲤鱼肾组织原代培养三周左右即可形成致密单层,传代细胞为5 - 6天左右传一次。培养细胞以成纤维样细胞为主,有少数上皮样细胞。
Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently , setting up to express system efficiently , suspending culture cell , can raise the cell density in the scale turn the production , strengthen the cell vitality , control cell to propagate level , extension cell culture period , increase the target protein of yield , raise product quality , simplification of produces technics , reduce production cost , then raising the efficiency that the scale turns culture 提要:采用无血清无动物组分培养基驯化高效表达细胞,构建高效表达系统,悬浮培养细胞,可以在规模化生产中,提高细胞密度,增强细胞活力,控制细胞增殖水平,延长细胞培养周期,增加目标蛋白的产量,提高产品质量,简化生产工艺,降低生产成本,进而提高规模化培养的效能。