端 Ⅰ名词1.(东西的头) end; extremity 棒的两端 the ends of a stick; 从这一端到另一端 from end to end; 岛的南端 the southern tip [end] of the island; 在它的最北端 at its northern extremity2.(事情的开头) beginning 发端 make a start; 开端 beginning3.(门类; 方面) item; point 变化多端 change constantly; most changeful; 举其一端 for instance; just to mention one example4.(原因) reason; cause 借端 use sth. as a pretext; 无端 without rhyme or reason; unwarranted5.(姓氏) a surname 端光 duan guangⅡ形容词(端正) proper; upright; straight 端坐 sit up straight; 品行不端 improper behaviour; misconductⅢ动词(平举着拿) hold sth. level with both hands; carry 端饭上菜 serve a meal; 端进两杯茶来 bring in two cups of tea; 端盘子 carry a tray; 有什么想法都端出来。 whatever you think, come out with it
c C major [minor] C大[小]调。 a C-spring C字形(承力)弹簧 (=Cee-spring)。 CCCL=350. C3丙等(的),体格劣等(的)。 (a C3 concert 第三流音乐会)。 C and A Pocket 〔美国〕无业游民做在上衣里用来装多余粮食的口袋 (Chicago and Alton pocket)。 C and S 【美军】整洁而严肃的 (=clean and sober)。
The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others , especially by lixinlal in naigin normal university of china , we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence , using the favorable condon of the ecoli . after cheked the result of synthesis by sequence , we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ) , and the fusion protein of thio - cmiv was highly expressed in soluble form 本研究的内容包括:一、在前人对抗菌肽cmiv研究的基础上,对n端和c端进行氨基酸保守变换,设计和合成了该基因,充分使用大肠杆菌偏爱的密码子,并将该基因5端与硫氧还蛋白基因3端融合,通过ptxfus表达载体获得较高可溶性表达(在15 sds - page胶上可见明显的表达蛋白带) 。
2 ) basis of upon studies , we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker . the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin 二、在以上研究的基础上,对cmiv抗菌肽的c端进行较大的改造,即将与肿瘤细胞过度表达的表皮生长因子受体( egfr )具有高亲和性的因子多肽tgf _ 3通过疏水柔性接头连接在抗菌肽cmiv的c端,设计完整的基因,并在大肠杆菌中利用ptxfus表达载体与硫氧还蛋白进行可溶性融合蛋白表达。