They used liquid and gas chromatography to fractionate the sample into its molecular constituents ; an electron beam then fragmented the separated molecules in a mass spectrometer to produce a spectrum indicating the basic chemical components 他们运用液相与气相层析法,将样本的组成分子分开,然后导入质谱分析仪,以电子束使已分离的分子断裂,进而产生出可识别基本化学组成的图谱。
( 3 ) the thickness change of the sample that is needed testing is converted into color change of test line in the colloidal - gold strip by gica . and then the sensing device of photoelectricity based on ccd is designed to convert the change of color into the change of the electric signal ( 3 )通过金免疫层析法将被测样本的浓度变化转化为金标试条测试线的颜色变化,在此基础上进行基于ccd的光电传感装置设计,进一步将测试线的颜色变化转换成电信号的变化。
In order to investigate the role of mannose receptor ( mr ) of human sperm , the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography . the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope , respectively . the mice were immunized with pmr and the antiserum was raised . after capacitation and induction of the acrosome reaction , the human spermatozoa and oocytes were incubated with the antiserum . then the sperm penetration assay was undertaken 为了进一步探讨人精于mr在精卵融合中的作用,本文采用改良后的甘露糖-琼脂糖凝胶亲和层析法分离纯化人精子mr ,并将提纯的人精子甘露糖受体( purifiedmannosereceptor , pmr )作用于去透明带的金黄地鼠卵母细胞,运用透射电子显微镜技术和罗丹明偶联的兵豆凝集素( tritc - lca )免疫荧光标记技术观察pmr对卵子的影响。
Methods tlc was used to identify the 6 kinds of crud drugs ( lycium barbarum , chrysanthemum morifolium , comas officinalis , paeonia suffruticosa , alisma orientalis , and rehmannia glutinosa ) , and all the established methods were used to examine the stability of three batches of the granules at mom temperature and acceleratin conditions 方法以薄层层析法鉴别杞菊地黄颗粒中枸杞子、菊花、山茱萸、牡丹皮、泽泻、熟地黄等6味药材;对该制剂3批样品及加速试验样品进行考察。
Extract the bursin from the bursa fabricius of chichens and ducks through the ultrafilter with loooda filter . ( the bursin of chickens - cbs . the bursin of ducks - dbs ) determine the major constitution by high - performance liquid chromatography ( hplc ) and thin - layer chromatography ( tlc ) . it is proved that there is bursin ( bs ) in two extractions . caculate the quantitation of bursin in the extraction of the bursa fabricius of chickens and ducks . it can be found through tlc that the quantitation of cbs in the extraction of the bursa fabricius of chickens is 1 . 436mg / ml and the quantitation of dbs in the extraction of the bursa fabricius of ducks is 4 . 035mg / ml . the result obtained through hplcis that the quantitation of cbs is 1 . 199mg / ml and dbs is 3 . 316mg / ml 采用超滤法从正常鸡、鸭法氏囊组织中提取分子量小于1000da的物质,分别以薄层层析法和高压液相色谱法检测其主要成分,证明两种超滤液中均有法氏囊活性肽(囊素三肽, bursin , bs )的存在,分别计算鸡、鸭法氏囊提取物中的囊素三肽的含量。薄层层析方法求得鸡法氏囊提取物中的bs含量为1 . 436mg / ml ,鸭法氏囊提取物中的bs含量为4 . 035mg / ml ;高压液相色谱的方法求得鸡法氏囊提取物中的贴含量为1 . 199mg / ml ,鸭法氏囊提取物中bs含量为3 . 316mg / ml 。
To control the quality of tpi , rp - hplc was used to determine the content of propanoic acid and proto - catechuic aldehyde in it . the results show that the average recovery rate was 98 . 01 % , 97 . 43 % and rsd = 2 . 75 % , 2 . 83 % respectively . this method is simple , accurate with strong reproducibility , and can be used for the quality control of danshen in any other compounded medications 利用反相高效液相层析法测定丹参素和原儿茶醛的含量以控制通脉粉针剂质量.结果表明,丹参素和原儿茶醛的平均回收率分别为98 . 01 % 、 97 . 43 % ,变异系数为2 . 75 % 、 2 . 83 % .本法简便、准确,重现性良好,可用于含丹参复方中药制剂的质量控制。
These instruments can be divided into three categories , chromatographic separation methods including gas chromatography , liquid chromatography and ionic chromatography , spectrometric methods including atomic absorption , ultraviolet / visible absorption , infrared spectrometry , atomic emission spectrometry and fluorescence method , and electroanalytical methods including potentiometry , coulometry and voltammetry methods 如层析法中的气相层析、液相层析及离子层析,光谱法中的紫外光可见光光谱法、原子吸收光谱法、红外线光谱法、萤光光谱法、放射光谱法及拉曼光谱法,及电化学方析方法如电位法、库伦法及伏安法等。
In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability , in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry , preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization 本研究用改良后的亲和层析法分离纯化mr , lowry法测定其蛋白质浓度,在精子穿透试验( spermpenetrationassay , spa )模型中定量研究其对精卵融合能力的影响并检测其细胞生物学活性;以已知浓度的pmr ( purifiedmannose - ligandreceptor )干预精子半透明带试验,观察用pmr预处理半透明带对精子与透明带结合的影响。