中英
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- Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control
选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p
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