The total dna of exoconjugants acquired dna degradation phenotype during electrophoresis , which suggested that the dnd gene cluster was heterologously expressed . the phz1358 was used sucessfully as a vector for gene replacement to identify the biosynthetic pathway genes for nanchangmycin in s . nanchangensis ns3226 . however , the gene replacement in another potential pks cluster has not succeeded till now 将利用phz1358构建的基因置换结构(孙宇晖,未发表)导入南昌链霉菌ns3226中进行了基因置换实验,协助完成了南昌霉素生物合成基因簇的克隆,但对另一潜在pks基因簇的基因置换实验目前还未能完成。
Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces . as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s . lividansbut seemed to be unable to replicates in streptomyces sp . fr - 008 , it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5 Scp2 *可以在变铅青链霉菌等许可宿主内复制并在许可宿主间有效转移,但似乎不能够在链霉菌fr - 008中复制,因此可能适合于在链霉菌fr - 008中通过进行基因置换及随后的链霉菌fr - 008和变铅青链霉菌间的接合过程来克隆完整的fr - 008生物合成基因簇。