A genome library of serratia marcescens cw - w - 90 - 3 strain was established by " shot gun " method and screened for phospholipase activity on lb - egg yolk plate 通过鸟枪法构建了粘质沙雷氏菌serratiamarcexcenscw - w - 90 - 3菌株的基因组dna文库。
Then , we find out the write clones through the chemis hib . during the working , three probes which we used are from the published sequences of spidroin . after this , a genie library of subclone of dragline was constructed 然后我们从构建蛛丝蛋白的亚克隆文库着手,采用鸟枪法测序的策略,取从蜘蛛cosmid文库中经过证实的阳性克隆scos - ds1 ,大,小为40kb左右,用cai , eco47 ,和hinf三种酶分别对其进行酶解分析。
The cdna library of major ampullate glands of araneus ventricousus was prepared successfully for the first time by using puc18 vector in the world . two genes encoding spider silk protein and one gene encoding no - silk protein were cloned by means of randomly picking colony ( bird gun ) 本研究在国内外首次构建了大腹园蛛( araneusventricosus )主壶腹腺( majorampullategland ) cdna文库,并以鸟枪法从中筛选出两个蛛丝蛋白和一个非丝蛋白cdna新基因。