Phylogenic tree from 29 strains were draw based on the ndv hn gene ( part segment ) , and the result is similar to that of f gene 根据29株ndvhn基因部分编码序列绘制ndv系统发育进化树,发现f及hn基因的分型结果大致相同。
Expression vectors of whole il - 6r gene and its carboxyl terminal partial gene sequence were constructed by pcr and gene recombination technique , respectively 6r基因全长和矮基端部分编码序列的表达载体,并导入大肠杆菌dhsq中,通过iptg诱导表达重组融合蛋白。
We obtained two positive clones after screening . sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis . the coding region was from n terminal amino acid 240 to c terminus 测序及序列比对结果表明二者序列相同,为ked样蛋白的部分编码序列,编码部分从n端第240位氨宋林夜:利用酵母双杂交系统筛选与g蛋白及cam相互作用的新型蛋白基酸至c末端,共207个氨基酸。