- point sequence
- sequence of points
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点序列的英文
例句与用法
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- Tracking join point sequences
跟踪连接点序列 - Approximate fixed point sequences for a finite family of lipschitz pseudocontractive maps
有限个李普希兹伪压缩映射近迫点序列的收敛性 - Approximate fixed point sequences and convergence theorems for lipschitz pseudocontractive mappings in banach spaces
伪压缩映射的近似不动点序列及其收敛定理 - Upon that an efficient thinning algorithm based on borderline ( etab ) is proposed which treats with individual pixels point in each step and picks up unilateral edge points sequence as the framework of a character image
算法每一步都针对单个的像素点作处理,并提取笔划单侧边缘点序列作为字符图像骨架。 - After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
F _ 1长38bp ， r _ 1长36bp ，其它片段均40bp长， f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定，获得300bp的片段。