Among 54 arbitrary primers tested , only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae 本研究经用54种随机引物对57种蟋蟀的rapd扩增分析得出以下结论: 1 )采用rapd技术,首次较系统地对中国蟋蟀总科7科26属共57种蟋蟀进行了系统学研究。在所试用的54种随机引物中筛选出可扩增出清晰可辨且稳定的rapd图谱的引物分别为:蟋蟀科9种、岭蟋科9种、树蟋科12种、结蟋科12种、蛛蟋科13种、铁蟋科13种、貌蟋科13种。
树: tree树蟋亚科: oecanthinae雪白树蟋: snowy tree cricket树蚺: cook's boa树蟾: tree frog树瘿: wart树蟾科: hylidae树鹨: anthus hodgsoni; olive-backed pipit; orienfnl tree pipit; oriental tree-pipit束: Ⅰ动词1.(捆; 系) bind; tie 束紧腰带 tighten one's waistband; 腰束皮带 wear a belt round one's waist2.(控制; 约束)control; restrain 无拘无束 without any restraintⅡ量词(用于捆在一起的东西) bundle; bunch; sheaf 一束稻草 a sheaf of straw; 一束美丽的黄玫瑰 a beautiful bunch of yellow rosesⅢ名词1.(聚集成一条的东西) beam 电子束 electron beam; 光束 light beam2.(姓氏) a surname 束皙 shu xi树槎: stump束 结间束: wenchebach树楂: crest