Establishment of a c57bl 6j es cell line by conditioned media of rat myocardial cells 用大鼠心肌条件培养基建立来源于c57bl 6j小鼠的es细胞系
Es cell line ; c57bl 6j strain ; conditioned media of rat myocardial cells ; chimera ; immortalization Es细胞系c57bl 6j小鼠品系大鼠心肌条件培养基嵌合鼠永生化
Using rt - pcr method , we found that rat myocardial cells expressed lif mrna , which might suppress es cells differentiation and stabilize x chromosome 采用rt - pcr方法,检测出大鼠心肌细胞有lif mrna的表达,这可能与其条件培养基保持es细胞未分化状态并使x染色体稳定有关。
Effects of parispolyphyllavar on the proliferation in human umbilicalartery smooth muscle cells by the conditional medium of the injured vascular endothelial cells induced by oxidized low density lipoprotein 蚤休水提液抗内皮细胞损伤条件培养基诱导脐动脉平滑肌细胞增殖作用的研究
Cercariae were collected , cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum . the schistosomula were cultured in conditional media up to 96 hours . the number of schistosomula was counted and the death rate was calculated 将条件培养基与童虫共培养, 96h内连续观察计数,计算童虫死亡率,与阴性对照比较,童虫死亡率最高的基因池进入下一轮筛选。