The construction scheme is briefly introduced herein , and furthermore , the diversion structures , the composite scheme consisting of open caisson group adopted to treat the overburden of the foundation for the stage 2 longitudinal concrete cofferdam and the diversion method of bottom outlet combined with the dam gap used for the stage 2 diversion are described as well 文中对该方案进行了简要介绍,并介绍了有关导流建筑物,以及二期纵向混凝土围堰堰基覆盖层采用了沉井群等综合处理方案和二期导流采用的底孔加缺口联合泄流方式。
Subconfluent cultures of rat were maintained in dmem / 10 % fbs , 24hrs before neuronal induction , media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ) , to initiate neuronal differentiation , the preinduction media were removed , and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media , 5hrs later , 40ul dmso was given to every hole containing 2ml each 分别取第5代和第13代的mscs ,以8x10 cm ‘浓度接种于六孔板中的盖玻片上,制备细胞爬片,每孔加zinl培养液。达到80融合时,更换新鲜培养液,并在培养液中加人终浓度为lrnm的p一流基乙醇,诱导24小时, pbs洗涤,而后换成无血清的培养液。