Notes on linkage mapping of gene by using three - point back - crosses 关于三点测交基因连锁作图的注记
An optimal sample for genetic linkage analysis with asp methods 方法进行基因连锁分析的一个最优抽样方案
To deal with dependent problems with epistatic interactions , experimental study of cga for nk landscape problem is given 对于不可作独立子问题分解的问题,则以基因连锁一般模型? nk模型进行了实例研究。
Microsatellite dna is among the most efficient class of molecular markers due to their hyper - variable and co - dominant nature with relatively high abundance and random distribution in the genome , and have been applied to a variety of fields including population differentiation , kinship analysis , linkage analysis , and evolutional and ecological studies 摘要微卫星dna由于具有高度多态性、共显性遗传、基因组中含量丰富且随机分布等特点,目前已成为最有效的分子标记之一,并应用于种群分化研究、血缘分析、基因连锁分析、进化以及生态学研究等许多领域。
2 . 5 ul of 10 x reaction buffer , 1 . 5 ul 25mm mgcl2 , 0 . 3 ul lomm dntp , 0 . 5 ul taqdna polymerase ( 5 u / ul ) , and lul ( = 20ng ) of primer were used in per reaction . each reaction was overlaid with one drop of paraffin oil . the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec , annealed at 37 1 min , extended at iv b 72 for 2 min and repeated the cycle 45 times , at last , extended at 72 ' c for lomin 等( 1995 )利用rapd标记区分美国东部一杂交地带的蟋蟀的两个姐妹种, allonemobiusfasciatus和a . socius ,并于1998年使用rapd和异型酶标ic做出了这两种蟋蟀的基因连锁图;国内田英芳、郑哲民( 20of )首次将rapd技术运用于蟋蟀总科的分子系统学研究中,采用2种引物对7种蟋蟀进行了基困组dna多态性研究,并应用upg问a法构建树状图,椎测系统发生关系