Amplified in e . coli xi - blue , the eluted phage in the third rounds was poured onto lb / iptg / xgal plate . we selected randomly 18 clones and amplified them , then confirmed positive clones by elisa 经双抗体(流感病毒的多抗和辣根过氧化物酶标记m13噬菌体抗体)夹心elisa鉴定的阳性克隆有12个,分别将其纯化、并进行dna序列分析。
The amplified dna fragments were inserted into pgem - t easy vector and sequenced . the dna fragment sequencing results from the two subspecies were compared to detect whether there was any difference 将pcr扩增产物克隆到pgem - teasy载体,进行dna序列分析,并用生物信息学方法比较东方田鼠长江亚种与指名亚种之间该序列的差异。
Method : a total of 101 isoniazid - resistant and 43 susceptible strains of mycobacterium tuberculosis were analyzed by pcr and sequence analysis of their katg , inha , ahpc , kasa , oxyr genes 方法对144株结核分枝杆菌临床分离株( 101株异烟肼耐药株及43株异烟肼敏感株) katg , inha , ahpc , kasa及oxyr基因进行pcr扩增及dna序列分析。
Conclusion the mtdna sequencing system using new primers was useful for forensic purpose . for difficult samples , dna typing with this system was especially available and practical , and has been validated in criminal cases 结论本课题所建立的线粒体dna序列分析体系是一种对法医疑难检材进行鉴别的有效、实用的方法,并且本方法已经在实际案件检验中得到验证。
Momp gene is amplified by using pcr technologyfrom dna of l2 trachoma chlamydia . the pcr products are recombined with vectors of pmd18 - t . more over , the recombinant plasmids are colonged . the dna sequence analysis shows the insert fragment is momp dna 本实验采用pcr技术,从l _ 2型沙眼衣原体dna中扩增出momp基因,与pmd18 - t载体重组行t a克隆,并进行dna序列分析,证明所克隆基因为mompdna序列。
dna: DNA = 1.deoxyribonucle ...序列: order; alignment; array; suc ...分析: analyse【统计】序列分析。: sequential analysis时序列分析: time series analysis相序列分析: facies sequence analysis序列分析: sequence analysis; sequencing; sequential analysis序列分析器: sequential analyser; sequential analyzer序列分析仪: sequenator; sequencer核酸序列分析: nucleic acid sequence analysis脉冲序列分析: pulse-train analysis毗邻序列分析: nearest neighbour sequence analysis; nearest neighbor sequence analysis时间序列分析: analysis of time series; time sequential analysis; time series analysis氨基酸序列分析: amino acid sequence analysis氨基酸序列分析器: amino acid sequence analyzer蛋白质序列分析: protein sequence analysis核苷酸序列分析: nucleotide sequence analysis金融时间序列分析: font-weight:boldanalysis of financial time series生物序列分析中心: technical university of denmark - center for biological sequence analysis时间序列分析法: time series analysis method紧邻序列分析,最近邻序列分析: nearest-neighbour sequence analysis气相蛋白质序列分析仪: gas phase protein sequencer时间序列分析的假定: hypothesis testing in time series analysis脱氧核糖核酸序列分析: dna sequencing液相蛋白质序列分析: liquid-phase protein sequencing