The ascites fliud titer of the other hybridoma s2 for further hybridization is 1 : 16000 by blood agglutination . the isotype of 2 - e4 is igg2a , and s2 is iggs 用于再杂交的另一细胞株为本室保存的分泌抗人a型红细胞单克隆抗体的杂交瘤细胞株s ; ,其血凝效价为l : 16000 。
Objection : this study is to prepare a kind of bispecific antibody of anti - hiv p24 antigen and anti - human rbc type a antigen , and to build a new way to detect hiv p24 antigen with this bispecific antibody 目的:制备抗hivp24和抗人a型红细胞双特异性抗体,建立检测hivp24抗原的新方法。
Therefore the hybrid - hybridoma can secrete not only bispecific antibody but also anti - human rbc type a monospecific antibody and probably anti - p24 monospecific antibody . the mixed antibodies purified by ammonium sulfate is further purified by p24 affinity chromatography . the unsp 这样,分离出双特异性抗体,并证实杂交杂交瘤除分泌抗p24和人红细胞的双特异性抗体外,还分泌抗p24单特异性抗体和抗人a型红细胞单特异性抗体。
Now the ways to detect hiv p24 include enzyme linked immunosorbent assay , immuno - chromatography , agglutination test , and so on . the way build in this experiment is an indirect rbc agglutination test , which is simple , fast , sensitive , and no pollution for the enviroment 目前检测p24抗原的方法有elisa 、免疫层析、凝集试验等,本实验是用人a型红细胞作指示剂,用间接凝集反应测定p24抗原,方法简便、快速,无污染,比较敏感。
This bispecific antibody companied with another anti - p24 monospecific monoclonal antibody can build a new way of indirect rbc agglutination to detect p24 antigen , and this new way is preliminarily applied to detect serial diluted p24 to determine the sensitivity 用双特异性抗体致敏人a型红细胞做指示剂,以抗p24单特异件单克隆抗体9a7做交联剂,建立间接血凝检测p24抗原的新方法,并初步应用于检测系列稀释的p24抗原以确定测定的敏感性。