In our previous study , yggg , speb were found in 2 . 8kb dna fragment that could bound era probe by using e . coli genome phage expression library to screen era binding protein 根据本课题组筛选era结合蛋白时获得的噬菌体中的插段包含大肠杆菌的ygggspeb基因,本研究分析了era与ygggspeb的相互作用。
Tanaka reported a novel method to isolate genes of drug - binding proteins directly from a cdna expression library by using the combination of drug and marker molecule 即利用标记分子与药物结合作为探针,直接从cdna表达文库筛选药物结合蛋白的基因并分离和体外表达,以便获取目的蛋白进行结构和功能研究。
So , the identification and characterization of its binding proteins will help to explore the physiological functions involved . to accomplish this goal , different methods were employed in this study 研究表明prpc发挥正常功能需要与另一蛋白结合协助作用,因此对其受体的分离和鉴定也有助于进一步探索其生理功能。
Cortactin , a novel member of filament actin binding protein family and the main substrate of non - receptor src protein kinase , plays an important role in the dynamic organization of cell cortex cytoskeleton 皮层蛋白( cortactin )是一种微丝肌动蛋白结合蛋白,它与肌动蛋白纤丝的侧面相结合,并直接参与皮层细胞骨架的组建。
Analysis of copper binding protein by sds - page , three main protein bands observed . the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc . 4 Sds page分析得到3条主要蛋白带,剪下这三条带进行胶内赖氨酸内切酶的消化,通过高效液相色谱分离肽段,选择性进行肽段的氨基酸序列测定。