- t向量
- t: 中世纪罗马数字的160。
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航 ...
- no vector: 无雷达引导
- s-t vector: s-t向量
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航线；航向指示。 3.【天文学】幅，矢径。 4.【生物学】带菌者[体]，传病媒介。 vt. 【航空】(对飞行中的飞机)指示航向。 vi. 【航空】电(磁)波导航。
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t vector中文什么意思
- t向量
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例句与用法
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- Main works and their important results are showed as following . firstly , the cdna encoding bullfrog gh is amplified by use of the total rna , extracted from adult bullfrog ' s pituitary , as the template , pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product , 660bp , was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method . after the purified cdna and pmd18 - t vector were ligated at 16 over night , the ligation products were transformed into e . coli strain dh5a and then the transformants were screened by clone pcr method
首先，以成体牛蛙的脑垂体总rna为模板，进行rt ? pcr扩增得到约660bp的特异性pcr产物带，切下琼脂糖凝胶中的特异产物带进行cdna胶回收，将cdna与pmd18 ? t载体进行t ? a克隆连接并转化到dh5 e . coli后，进行菌落pcr 、质粒酶切鉴定，筛选出阳性菌株，测序结果经blast分析，与已报道的牛蛙生长激素基因高度同源，证实此阳性克隆为牛蛙生长激素基因转化子，命名为pbfgh 。 - The total rna was extracted from human normal kidney tissue and the cdna fragment of hnadcs was produced by rt - pcr , then was purified and inserted into pgem - t vector . after dna sequenc - ing , pgem - hnadcs was digested with ecor i and sal i , and ? the dma fragment was subcloned into the ecor i and sal i sites of the fusion expression vector ( pgex - 5x - 1 )
从正常人肾组织中提取总rna ， rtpcr扩增hnadc3抗原表位区cdna片段，产物克隆至pgem吓载体，测序正确后，将hnadc3抗原表位区dna片段再次亚克隆至pgex融合表达载体，构建重组质粒pgex十nadc3 。 - Using pcr technology , a 2 . 4kb dna fragment , part of tryptopanase operon , containing a promoter , a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e . coli k - 12 , was cloned to pmd18 - t vector . the dna sequence is the same as which was published on science
为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个dna片段克隆到pet28c质粒的bamhi和hind位点上，使该片段受t7rna聚合酶的启动子控制，然后转化噬菌体de3的溶源菌bl21 ( de3 ) 。 - Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr . the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector , respectively . the recombinants were identified by restriction enzyme analysis and dna sequencing , iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
应用smartpcr试剂盒和简并引物从人皮肤角质形成细胞cdna中扩增到长分别为24obp和13obp左右的2种片段，将它们插入pmd18一t载体，用酶切法初步筛选阳性重组子pm . hrabl和pm一hrabs ，对阳性重组子进一步作测序鉴定。 - The pcr products were purified and linked with pmd 18 - t vector respectively . the positive recombinants were selected and digested with double restriction enzymes respectively . goat fsh - a and p subunit genes which had sticky ends were purified and linked respectively with pf which also had the corresbonding sticky ends and were purified
将所得的山羊fsh - 、亚基基因分别用加相应限制性内切酶酶切位点的引物大量扩增，回收后与pmd18 - t连接，筛选出阳性重组子，大量双酶切回收获得带粘性末端的山羊fsh - 、亚基基因，将它们分别与同两个酶大量双酶切回收获得的带粘性末端的pf质粒进行连接，筛选阳性重组子。