Scolopendra multilan is a common species belonging to scolopendromorpha , chilopoda . the sequence of scolopendra multilan mtdna determined is 11700 bp in length , about 70 % of the complete sequence . the sequenced portion contains 2 rrna genes , 1 a + t - rich region , 8 protein - coding genes and 18 trna genes 本文对少棘蜈蚣的线粒体基因组进行了研究,已经测定的序列长11700bp ,约为全长序列的70 ,包含了2个rrna基因、 1个at富含区、 8个蛋白基因、 18个trna基因。
Rna polymerase i synthesizes the three largest rrnas . rna polymerase ii mainly produces mrna encoding proteins and most of sn rnas , and rna polymerase iii makes 5s rrna and trna , as well as a few small nuclear rnas . the transcription sites of the polymerases have been studied since early 1980s 真核生物细胞核中有三种rna聚合酶,即rna聚合酶、和,它们分别转录产生不同的rna ,其中rna聚合酶( pol )转录合成45srrna前体;聚合酶( pol )转录合成mrna前体及大多数snrna ;聚合酶( pol )转录合成5srrna 、 trna和一些小分子rna 。
Robustness of our results was confirmed by high bootstrap support of all nodes in the trees . this result contradicts the batrachia hypothesis ( a salamander + frog grouping ) , and is consistent with bolt ' s hypothesis ( 1991 ) basing on the morphological data . the result was also supported by previous molecular studies based on mitochondrial and nuclear rrna data 这个结果与蛙类假说是相矛盾的,与bolt ( 1991 )中国泽蛙线粒体基因组结构及种群系统地理学研究在形态学基础上提出的绒蝶类和蚓螺类为姐妹群关系的假说相一致,并得到建立在线粒体和核trna基因数据基础上的许多分子研究的支持。
The partial sequences of the mitochondrial co i , co ii and trna gene of the adults and larvae of 4 species from lepidostomatidae and the adults and larvae of apsilochorema unculatum and a . hwangi were sequenced and compared in this study . in the sequences obtained ( 1718 ~ 2239bp ) , a % + t % was about 69 . 2 % , 267 nucleotide sites were substituted in apsilochorema and 266 nucleotide sites were substituted in lepidostomatidae . at bias in the third codon position site was much higher than the other two sites , reaching about 81 . 9 % {的比对分析表明:在coi区的一45一个比对位点中,有1260个保守位点, 191个变异位点,其中98个转换位点, 93个颠换位点;发生在密码子第三、第一和第二位点的变异分别为78 % 、 18 %和4 % ;种内序列歧异度为o一0 . 9 % ,而种间为一4 %一24 % 。
The morphological diagnostic characters for many mature and early instar larvae are still lacking in china . partial sequences of the mitochondrial cytochrome oxydase i and ii and a transfor rna ( co i co ii and trna ) gene of the adults and larvae from caddisflies were . lepidostomaflavum , l . fui , l . arcuatum , paraphlegopteryx morsei , apsilochorema unculatum and apsilochorema hwangi , and the larval and adult stages of these species were sequenced and associated 采用dnastarpackage中的editseq软件进行序列编辑、 orf查找;采用clustalx软件进行序列比对( alignment ) ;比对结果输入mega2 . 1软件计算各样品间的遗传距离,并基于kjmura2 - parameter模型,用邻接法( neighbor - jojning , nj )构建系统发生树,通过自展( bootstrap1000次)检验获得系统树分支的置信度。
tRNA {略} : transfer RNA トランスファーRNA、運搬{うんぱん}RNA、転移{てんい}RNA
trna meaning: Noun: tRNA RNA molecules present in the cell (in at least 20 varieties, each variety capable of combining with a specific amino acid) that attach the correct amino acid to the protein chain tha...trna перевод:транспортная рибонуклеиновая кислота