- t向量
- t: 中世纪罗马数字的160。
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航 ...
- no vector: 无雷达引导
- s-t vector: s-t向量
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航线；航向指示。 3.【天文学】幅，矢径。 4.【生物学】带菌者[体]，传病媒介。 vt. 【航空】(对飞行中的飞机)指示航向。 vi. 【航空】电(磁)波导航。
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t vector中文什么意思
- t向量
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例句与用法
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- [ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ) , full length fasl gene is detected by rt - pcr . using the cdna as template , . the extracellular domain of fasl ( fasl - ecd , 127 - 278aa ) is amplified by pcr . the pcr products are directly cloned into t vector pmd - 18t
L方法采用新鲜人黑色素瘤细胞( a375 ) ，抽提该细胞的总rna ，进行rt一pcr反应分析a375内fasl全长编码基因的转录表达，以a375细胞cdna为模板，用pcr产物直接克隆法扩增人fasl一ecd (人fasl胞外区)的编码基因，即127一278位氨基酸残基，而后将pcr产物直接克隆于pmd一1st载体中，获得重组质粒pmd一t - fasl一ecd ，进行dna测序。 - In this article , the author amplified dna sequences encoding the mature peptides of human intact and truncated igf - 1 gene from human genomic dna by the new way of alternative pcr established and named as exons - piecing together method by this laboratory . the igf - 1 and des ( l - 3 ) igf - l gene were cloned in pgem - t vector and then subcloned in expression plasmid pgex - 3x . two recombinant constructs known as pgex - 3x - igf - l and pgex - 3x - des ( l - 3 ) igf - l were transformed to e . coli dh5 a respectively
我们利用外显子拼接法从人的基因组dna中扩增了编码完整型及截短型人igf - 1基因成熟肽的dna片段，并将其克隆至pgem - t载体中；经测序证明正确后，又构建了表达载体pgex - 3x - igf - 1及pgex - 3x - des （ 1 - 3 ） igf - 1 ，在大肠杆菌中进行了表达研究。 - The purified production was cloned into pmd18 - t vector . the cloned plasmids were transformed into jm109 . the specific recombinant plasimid was identified by molecular weight , pcr and restriction endonuclease analysis . the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation
经琼脂糖电泳检测，将大小与预计分子量一致的片段纯化后连接到pmd18 - t载体中，再转化到jm109感受态细胞，得到的转化子经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆，结果表明，得到的阳性重组子中含有a节段全长基因，插入到载体中的方向正确。 - Then , the plasmid was transformed into jm109 . the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence . test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program
利用rt - pgr技术，设计一对引物，对田间采集的经鉴定含pstvd的阳性样品进行全序列扩增，并将所得产物纯化回收，连接到pmd18t - vector中，并转化至大肠杆菌jm109中，挑选白斑进行双酶切( saci / ecorv )鉴定证明插入片段为359bp大小，进行序列测定，所得克隆基因为pstvd全序列负链，大小为359bp 。 - On the bases of designing a primer pair , we obtained the coding domain sequence of rbp by pcr from cdna library . then the gene was cloned into pgem - t vector , the dna sequencing showed that the cloned gene was in agreement with the reported sequence . then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp . in order to expresse rbp in procaryotic cell efficiently , the recombinant plasmids was introduced into e . colidhs a straints . expression of rbp was induced by temperature induction
本实验在合成该蛋白基因上下游引物的基础上，利用pcr技术，从人睾丸cdna库中钓取目的基因，并克隆于pgem - t载体中，经序列测定证明与文献报道基本一致，再将目的基因从重组克隆质粒中亚克隆于pbv220原核表达载体中，转化宿主菌，经温度诱导后，进行sds - page电泳分析，发现在约21kd位置上出现了一条明显的蛋白带，与预期相符。