- t向量
- t: 中世纪罗马数字的160。
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航 ...
- no vector: 无雷达引导
- s-t vector: s-t向量
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航线；航向指示。 3.【天文学】幅，矢径。 4.【生物学】带菌者[体]，传病媒介。 vt. 【航空】(对飞行中的飞机)指示航向。 vi. 【航空】电(磁)波导航。
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t vector中文什么意思
- t向量
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例句与用法
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- Part two : studies ; l . the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene . the amplification band was observed in both male and female dinodon refozonatum , whose length was consistent with that of human sry gene , which about 220bp . the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human , and there was a few differences between male and female dinodon refozonatum . 2 . using a pair of degenerate primers based on the conservative region , hmg - box , of human sry gene , six different fragments were amplified from either female or male dinodon refozonatum , then cloned by using pmd18 - t vector and sequenced
结果显示： ( 1 )赤链蛇基因的扩增片段与人sry基因扩增片断大小相同，为220bp左右； ( 2 )雌、雄赤链蛇sox基因的扩增片段大小虽然与人的相同，但其单链迁移率与人的有较大差异，而且雌雄个体间有明显差异，预示该基因的dna序列在雌雄个体中可能有差异； 2 、参照人sry基因hmg - box保守区的序列，又设计一对兼并引物，扩增了赤链蛇的sox基因，并对扩增产物进行了克隆和测序。 - The pcr products were purified and cloned into pucm - t vector then vector pp - rd29a was constructed . by white - blue screening , colony pcr and enzyme digest analysis , the result showed the plasmid containing p - rd29a fragment was transferred into e . coli xl 1 - blue . the sequencing of the single recombinant bacterium containing the inserted p - rd29a fragment was carried out by baiasia bio - engineering co . ltd
纯化的目标产物经taqdna聚合酶加a碱基后，采用taclonging方法克隆到pucm - t载体中，得到重组质粒pp - rd29a ，转化大肠杆菌xl _ 1 - blue ，挑选白色的重组子菌落进行colonypcr筛选和质粒的酶切验证。 - The chi gene fragment was amplified by means of rt - pcr , using flower mrna of saussurea medusa maxim , as template , and a pair of specific oligonucleotides ( according to the chi gene sequence , af509335 ) as primer , and cloned into pucm - t vector with ta connection . the vector was named pucm - chi
Chi基因的正向和反向植物表达载体的构建用ecor和sal双酶切p ~ ( ucm - chi )和中间载体ptriplex2 ，回收、连接目的片段与载体，构建中间载体p ~ ( tripchi ) ，选择正向重组质粒。 - A complete cdna of annfaf expressed specifically in strawberry fruit was cloned via rt - pcr and was subcloned to pmd18 - t vector to construct the recombined pmd18 - t ( annfaf ) vector . the inserted fragment was sequenced and the character of deduced amino acids was analyzed to obtain the antiserum
)为试材，从其完熟果实中提取总rna ，以genbank中发表的annfafcdna序列为依据，利用rt - pcr技术克隆得到草莓果实特异表达基因annfaf的cdna序列，将其克隆到pmd18 - t载体中构建成重组克隆质粒pmd18 - t ( annfaf ) 。 - The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector . the sequencing results showed that pap gene had 99 . 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ) . the iptg - inducible expression vector containing the pap gene was constructed and transferred into e . coli bl21 ( de3 ) - plyss
将缺失型pap基因克隆到植物表达载体pbi121中，通过液氮冷冻法将重组质粒转入农杆菌lba4404细胞中，然后采用叶盘法，在该农杆菌的介导下将pap基因导入普通烟草中，经过卡那霉素抗性筛选，最后获得了转pap基因的工程烟草植株，摩擦接种烟草花叶病毒( tmv ) ，与非转基因烟草相比，能够推迟症状表现达2月之久，说明pap基因能够在其它植物体内产生有活性的高抗病毒的蛋白质。