- t向量
- t: 中世纪罗马数字的160。
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航 ...
- no vector: 无雷达引导
- s-t vector: s-t向量
- vector: n. 1.【数学】向量，矢量，动径。 2.【航空】飞机航线；航向指示。 3.【天文学】幅，矢径。 4.【生物学】带菌者[体]，传病媒介。 vt. 【航空】(对飞行中的飞机)指示航向。 vi. 【航空】电(磁)波导航。
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t vector中文什么意思
- t向量
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例句与用法
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- Clone human wnk4 full length cdna into pgem - t vector human kidney total una was extracted and used as template to amplify wnk4 full length cdna with the forward primer ( 7 - 27 ) and reverse primer ( 3808 - 3833 ) with the long template expanded pcr system kit
5 .原位杂交以小鼠肾脏总翩a为模板，扩增wnki基因外显子1 ， 24一28 ，以a片段和wnk4基因外显子13一16片段，纯化后连接在pgem一t载体上。 - 2 . cloning of the pcr products the pcr products were purified by agarose gel electrophoresis and was ligated with pucm - t vector . by the method of pcr and enzyme digest analysis . the result shows that the plasmid containing cpti gene was transferred into e . coli dhso
Pcr产物的克隆采用a / t克隆法，将pcr产物经琼脂糖凝胶电泳纯化回收后用t4连接酶与pucm - t载体连接，构建成克隆载体puc - cp ，转化大肠杆菌dh _ 5 。 - Pcr methodology was adopted for cloning of chitinase encoding genes . based on the sequences of chitinase gene from genebank , the primers for chitinase gene amplification were designed . pcr fragment was ligated with pmd18 ~ t vector and transformed into e . coli dh5 a
尽管同源性比较低，但酶活性检测发现该基因片段具有几丁质酶活性，认为此pcr片段含有几丁质酶编码基因的全序列或部分序列，此基因片段是一个新基因或基因片段。 - The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 - After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
F _ 1长38bp ， r _ 1长36bp ，其它片段均40bp长， f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定，获得300bp的片段。