Precursor cells from different lineages express different subsets of surface molecules , many of which are now difined by cluster of differentiation ( cd ) antigens . cd antigens associated with the plasma membranes of leukocytes may be molecules involved in a variety of functions 蛋白芯片可以将蛋白质以阵列的方式固定在经化学或生物酶处理的表面上,也可将受体蛋白、抗体等固定在表面上,未知分子可以利用与芯片不同位点亲和力来分析。
We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity , as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective . part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system 曰补体受体及其相关分子在modc上的表达不同分化阶段的单核细胞衍生性dc ( monocytes一deriveddc , modc )的诱导:将新鲜分离的单核细胞mo ,在含有gm一csf和工l一4培养体系中诱导5一7d ,即分化为未成熟modc ;对培养至sd的未成熟modc ,用tnfa刺激zd ,即分化为成熟modc ;此时再用lps刺激24h ,即为活化的modc 。
This concern , recognized by a 10 - year - old , is very real : the potential of gamma - secretase as a therapeutic target is tempered by the fact that this enzyme plays a critical role in the maturation of undifferentiated precursor cells in various parts of the body , such as the stem cells in bone marrow that develop into red blood cells and lymphocytes 这个连10岁小孩都注意到的顾虑,是再实际不过的事了:虽然分泌酶可能是个治疗标的,但我们却不能不注意一个事实:这个酵素对身体各处未分化的前驱细胞(像是可分化成红血球与白血球骨髓干细胞)的成熟,扮演著关键角色。
Day 4 embryoid body ( 4deb ) cells were derived from es cells and then induced with mbmec - cm into hematopoietic precursor cells . immunocytochemistry staining and flow cytometry were adopted to observe the antigen expressions . rt - pcr method was used to detect the expressions of hematopoietic genes 在诱导小鼠es细胞向造血细胞分化的实验中,我们采用先将小鼠胚胎干细胞形成4天拟胚体( day4embryoidbodies , 4debs ) ,再用骨髓内皮细胞条件培养液诱导4天拟胚体细胞生成造血干祖细胞。