We used fission yeast schizosaccharomyces pombe ( s . pombe ) , an unicellular eukaryotic organism , as research material . electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ) , we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell . flow cytometry provided a way of determining the relative dna content of populations of fission yeast 本文以单细胞的真核模式生物裂殖酵母( schizosaccharomycespombe )为研究材料,通过激光扫描共聚焦显微镜观察酵母细胞胞质内游离ca ~ ( 2 + )的分布及相对浓度,以及不同周期时相细胞中gfp - cam的荧光强度变化,并采用细胞流式法对酵母细胞的相对dna含量进行测定以确定细胞所处周期时相。
The lung tissue for immunohistochemitry and laser scanning confocal microscopy were fixed and embedded . the morphological alteration of pulmonary neuroendocrine cells which stain for calcitonin gene - related peptide ( cgrp ) , serotonin ( s - ht ) and luteinizing hormone ( lh ) were studied . the results of these were dealed with computer image analysis and statistical treatment 肺组织取材后经固定、梯度酒精脱水、包埋、连续切片后,应用免疫组织化学方法、透射电镜及激光扫描共聚焦技术观察了降钙素基因相关肽( cgrp ) 、五羟色胺( 5 - ht ) 、黄体生成素( lh )阳性细胞的随龄变化,并对实验结果进行了计算机图像分析和统计学处理。
laser scanning confocal microscopy meaning:[Medicine] A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light so...