The expression vector pse380 - / iy / was constructed and transformed into e . coli dh5a , expressing hyl gene by adding iptg into the broth . the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0 . 1 m acetate buffer solution ( ph4 . 0 ) 经转化大肠杆菌dh5a和iptg诱导表达后用sds - page电泳分析,获得一条约120kda的表达条带; iptg诱导表达后提取原生质膜测定透明质酸分解酶活力,表明该hyl片段的产物能够在体外分解细菌来源的ha 。采用两种策略灭活hyl基因。
In c . deserticola , the activities of invertases were low and the activities of sucrose synthase and sucrose phosphate synthase were high , and the activity of sucrose synthase was higher than that of sucrose phosphate synthase , which appeared , which was manifested in that in c . deserticola , the activities of clastic enzymes were higher than that of synthases and the higher activities of clastic enzymes improved sucrose breakdown thus enhancing the transfer of sugar from h . ammodendron to c . deserticola 肉苁蓉体内转化酶活性较低,而蔗糖合成酶和蔗糖磷酸合成酶活性较高,且蔗糖合成酶活性高于蔗糖磷酸合成酶活性,表现为肉苁蓉中的分解酶类活性高于合成酶类活性,较高的分解酶类活性促进了蔗糖的分解,从而促进了糖分由寄主梭梭向肉苁蓉的不断转移。