2 . using a pair of degenerate primers based on the conserved region , hmg - box , of human sry gene , eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced 2 、参照人sox基因设计了一对兼并引物,扩增了虎纹蛙的sox基因,并对扩增产物进行了克隆和测序。
To device a primer pairs and amplify the full length pstvd by rt - pcr , the positive rna extraction from tuber sample was used as template . the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector Cdna核酸斑点杂交反应( nash )检测pstvd方法准确、灵敏度高,一次检测样品数量多,且对于异地样品检测非常方便,是以往其它检测方法的有效补充。
Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis 利用genbank中已经登录的其它植物中该酶基因的保守序列,设计一对简并引物,采用rt - pcr技术,从茶树扩增出208bp的cdna片段。
The tmek2 and tmek2mut was amplified by pcr and then cloned to pmd 18 - t vector . to express the genes in plants , the coding region of tmek2 and tmek2mut were placed between the 35s promoter and nos terminator of pibl , respectively 载体构建的过程包括目的基因的pcr扩增、目的基因连接到pmd18 - t载体、目的基因连接到中间表达载体pib1 、目的基因及启动子、终止子连接到表达载体pbin19 。
Designing a specific primer pair based on the sequence of the gene ubia in e . coli mc4100 , a dna fragment was amplified from the genomic dna of e . coli mc4100 by pcr and then was cloned into pucm - t vector . the cloned fragment was sequenced 根据e . colimc4100的ubia基因全碱基顺序,合理设计引物,以e . colimc4100基因组dna为模板进行pcr扩增,将pcr产物克隆于pucm - tvector后对其进行测序鉴定。