The phylogenetic tree was constructed based on sequence comparison and analysis . the result showed that c strain derived from rabbit spleen tissue , c strain adapted to sk6 cell , shimen virulent strain all belonged to group 1 . 1 , and the prevalent virulent strains belonged to subgroup 2 . 1 or subgroup 2 . 2 . this indicated there were at least two subgroups , which were prevailing in china , and the prevalent virulent strains were diverse far from c . strain vaccine virus 通过序列比较和系统发生关系分析发现: c -株兔组织毒、 c ?株细胞毒和中国50 60年代流行的石门强毒株同属于组群1 ( group1 ) ;近期猪瘟流行毒株均属于组群2 ( group2 )的两个不同的亚组群( subgroup2 . 1和2 . 2 ) ,表明我国目前流行毒株至少有2个亚组群。
With the strengthening human ’ s impact to the nature , the earth land surface 1s exderiencing profound change land use land cover change has become one of the most important study areas of global changes urbanization and industrialization are major types of land use change in china today , which will certainly have a deep effect on the land use structure and food safety in this paper , multi phases satellite image including corona , tm , etm + data which redresent the information of yixing of 1 966 , 1 984 , 1 992 and 200 1 , respectively were dealt with multi - bands composition , precise rectification , interpretatlon ma ‘ k establishment , computer assistant visual interpretation by the methord of multi 。 phase - sequence comparison and integrated technology of rs and gis vetor maps of land use of 1 984 , 1 992 and 200 1 are attained by interpreting images above . by the data above and the image of corona of 1 966 , vector maps of expanding towns ot yixing city from 1 966 to 2001 are acquired 城市化、工业化已经成为当前我国土地利用变化主要表现形式,它必将对我国的土地利用结构和粮食安全产生深远的影响。本文借助遥感和gis技术,采用多时期连续对比法对fm , etm + pan等多源卫星遥感影像进行土地利用人机交互解译,获得了高精度的宜兴市1984 、 1992 、 2001年三个时期l : 5万土地利用图。利用以上各时期的土地利用数据库并结合1966年corona卫星影像资料建立了1966 、 1984 、 1992和2001年四个时期的城镇建设用地数据库。
2 . sequence comparison of alpha - amylase gene of bombyx mori and bombyx mandarina the alpha - amylase gene structures of bombyx mori and bombyx mandarina are very similar , both comprised of five exons and four introns , with comparable length . the mean at contents of exons and introns are not much different 家蚕和野桑蚕-淀粉酶基因序列比较分析家蚕和野桑蚕-淀粉酶基因具有相似的基因结构,都包含5个exon和4个intron ,各内含子、外显子长度在家蚕和野桑蚕间较类似,外显子和内含子at的平均值差异不大。
It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ) , and the sequence were cccccc in f48e9 strain , and tcccac in zj1 strain . in order to test if insertion of this six nucleosides is related with the virulence of nd , two primers were designed to amplify the same fragment of another ten ndv strains . the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain . this suggested that the six nucleosides insertion might have relationship with the ndv virulence . compared with all known sequence of ndv . there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain 通过rt - pcr方法扩增获得了另外10个背景清楚毒株的np - p基因间隔区片段,将这些序列与f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相应序列进行了比较,结果在参比的16个ndv毒株中在该区段中除了有多个点突变外,个别毒株有碱基插入和缺失,所有以lasota株为代表的弱毒株均无6碱基的插入,而以f48e9株为首的强毒株均有此6碱基的插入,但有一个中等毒力的毒株dp没有6碱基的插入,不过它的基因序列和lasota的几乎相同,对于所克隆到的基因的代表性还有待确定。