- 反相高压液相层析
- reverse: vt. 1.使颠倒，使倒转，使反转；使翻转；翻(案)。 ...
- phase: n. 1.形势，局面，状态；阶级。 2.方面，侧面。 3 ...
- normal-phase hplc analysis: 正相hplc分析法
- reversed-phase hplc: 反相高效液相色谱法
- phase reverse: 相位调换
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reverse phase hplc中文什么意思
- 反相高压液相层析
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例句与用法
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- Secondly , determination of the antimicrobial activities of synthetic hpab - . i ) the mature peptide of hpab was synthesized by fomc method and purified by reverse phase hplc . the molecular weight of synthesis peptide was 4334 . 96da characterized by mass spectrometry , highly agreed with the theoretical mw of hpab - 3
2 . hpab一p抗菌活性初步鉴定:采用固相法合成了hpab一p成熟肤，经即一hplc纯化后用质谱测定合成肤的分子量为4334 . % da ，与其理论分子量相符。 - Both hainantoxin - iv ( hntx - iv ) and . hainantoxin - v ( hntx - v ) w ere peptide toxins isolated from the venom of the spider seleconosmia hainana by means of ion - exchange chromatograph and reverse phase hplc . hntx - iv , 3989 da , contained 35 amino acid residues with three disulfide bonds , while the molecular weight of hntx - v was 3972 da and had three disulfide bonds , too
海南捕鸟蛛毒素- ( hntx - )和海南捕鸟蛛毒素- ( hntx - )是从海南捕鸟蛛粗毒中经阳离子交换和反相hplc柱层析分离出来的两种神经毒素， maldi - tof质谱鉴定前者相对分子质量为3 ， 989da ，含有三对二硫键，由35个氨基酸残基组成；后者相对分子质量为3 ， 972da ，与前者只相差16个质子，也含有三对二硫键。 - The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。 - A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc . hwtx - v has 35 amino acid residues , with the molecular mass 4111 . 4da . the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh , which consists of 6 cys , formed three pairs of disulfide bridges
本文报道从虎纹捕鸟蛛( selenocoimahuwena )粗毒中，结合阳离子交换和反相高效液相色谱分离纯化出一种昆虫毒素，命名为虎纹捕鸟蛛毒素- ( huwentoxin - ， hwtx - ) ；其天然突变体( themutantofhuwentoxin ， mhwtx - )也同时纯化出来。