Here , aba - induced h2o2 generation and their roles in inducing stomatal closing were investigated by epidermal strips bioassay , laser scanning confocal microscopy and patch clamp with ios5 and its wild type plant as materials . there were no clear differences between wild type and ios5 in the density , shapes and aperture of their stomata 本文以los5及其相应野生型为材料,采用表皮条生物分析、激光扫描共聚焦显微技术及膜片钳技术研究了nacl胁迫条件下, aba诱导保卫细胞内h _ 2o _ 2的产生及二者在诱导气孔关闭中的作用。
This new method will bring significant developments in studying the principles of stomatal movement , and other quick movement in plants , c ) guard cells are incubated with ph dependent fluorescent chemical probe " bcecf am " and excited at 488nm , the fluorescent emission ratio method ( 520nm / 640nm ) is employed with laser scanning confocal microscopy , about 0 . 4 ph unit increase in guard cell vacuoles is observed during stomatal closure that is induced by aba 本发展为保卫细胞与其它小细胞液泡的进一步研究提供了新思路。 c )本工作通过激光共聚焦显微术配合ph荧光探针bcecfam的单激发( 488nm )双发射的荧光比值法( 525nm 640nm )观察到,用aba处理的表皮条上的开放态气孔在关闭过程中其保卫细胞液泡内ph有一约0 . 4单位的上升。
So it is necessary to examine calcium activity and distribution in nerve cells . a way of visualizing intracellular ca2 + in three dimensional was established by using laser scanning confocal microscopy ( lscm ) and computer visualization technique in this paper . based on this way , which includes cell culturing and dyeing , confocal microscopy optimizing , confocal data preprocessing , 3d visualization of ca2 + by computer , we investigated the ca2 * distribution in cultured hippocampal neurons under different objectives 本文通过激光扫描共聚焦显微技术和计算机三维可视化技术建立了一套神经细胞内钙离子分布三维可视化的方法,包括细胞的培养和染色、显微镜参数的优化、共聚焦数据的预处理、针对钙离子的三维可视化方法的实现,为胞内钙离子作用机制的研究提供直观的手段。
The homepage provides the information of research interests , details of seminar schedules ( date , speaker , title ) , course descriptions , research facilities ( includes cell & molecular imaging facility , laser scanning confocal microscopy and so on ) , and links to university of north carolina medicine department , centers and programs , curricula , related center and program ( includes bowels center for alcohol studies , lineberger comprehensive cancer center , cell and molecular biology trainning program , center for gastrointestinal biology and disease , department of ophthalmology and so on ) 中文简介:查珀尔希尔北卡罗来纳大学医学院细胞和发育生物学系的主页提供研究方向信息,讲座日程安排的详情(日期,发言者,标题) ,课程描述,研究设施(细胞、分子成像设备,激光扫描共焦显微镜等等) ,与北卡罗来纳大学医学系,中心,项目,课程,相关中心与计划(酒精研究内脏中心,林内贝格综合癌症中心,细胞与分子与生物训练计划,肠胃生物疾病中心,眼科系)的链接。
Based on epidermal strip bioassay , microinjection , patch - clamp and laser scanning confocal microscopy in the experiments , we provided the first evidence that map kinases , including mek1 / 2 or p38 / hog1 , plays an important role i n aba - or sa - induced h2o2 signal initial , amplification and specific targeting in response to stimuli in guard cells . aba - or h2o2 - induced vicia faba stomatal closure . was inhibited or reversed by the specific inhibitor pd98059 of mek1 / 2 ; the guard cells were pre - incubated or - microinjected by 10 umol l - 1 pd98059 , aba could not enhance the fluorescence intensity of h2o2 probe dichlorofluorescein ( dcf ) 在对照实验中, aba诱导荧光迅速增高;单独的pd98059 、 pd98059和aba共同处理气孔时,保卫细胞内h _ 2o _ 2探针荧光强度没有增高;将pd98059注射进入其中的一个保卫细胞,再以aba处理,使得两个有同样荧光基础的保卫细胞荧光强度对比强烈;将pd98059显微注射进入已被aba诱导dcf ( dichlorofluorescin )荧光强度升高的气孔保卫细胞,荧光强度下降,而没有被注射一边的保卫细胞中的dcf荧光强度不变。
laser scanning confocal microscopy meaning:[Medicine] A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light so...