insertion site是什么意思_insertion site中文

insertion site中文什么意思

插入位点,克隆位点

insertion: n. 1.插入；记入；刊登。 2.插入物；插入句；插入广 ...
site: n. 1.地点；位置；地基。 2.场所，现场。 3.遗址 ...
insertion: n. 1.插入；记入；刊登。 2.插入物；插入句；插入广告；插锈，补绣。 3.【动、植】着生(点)。 4.【电学】嵌入，介入。 5.【医学】(肌肉的)附着。 the insertion of muscle 肌附着，肌止端。 adj. -al
insertion (): 引入操作符
a site: 氨基酸附着部位; 氨酰基部位

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And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8
试验研究设计并合成了由40和44个碱基的寡聚脱氧核苷酸组成的染色体爬行接头，在接头序列和测定的f片段近tn5的序列上，设计了2对染色体爬行用的pcr引物，从b8菌株中提取基因组dna ， bamhi酶切，与染色体爬行接头连接，依次用2对引物进行pcr ，扩增出239bp产物，经克隆、测序，发现其中18bp为扩增的相应于f片段在b8f菌株tn5插入位点对面的序列，其余则为f片段728bp序列的一部分，为进一步进行染色体爬行，克隆和测定整个b和f基因，揭示阳菌株的拮抗分子机制提供了技术资料贮备。
In addition , molecular experiments indicated that resistance was not relevant to copy number or to insertion sites , but instead to the repeated gfp structure and to methylation of genomic dna . pvx vectors were constructed that contained the s6 gene of rbsdv vi and the 14 kd fragment of bnyvv rna2
分子检测结果显示， gfp3植株中普遍存在gfp基因的沉默现象，且gfpdna的甲基化程度明显高于gfp1植株，说明这些植株对于病毒的抗性与目的基因的插入位点及拷贝数和无关，但与串联重复的gfp基因结构形式密切相关。

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insertion site中文什么意思

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