Blast in genbank indicated that it is novel gene named par - 1 . the characteristics of the protein coded by this gene were analyzed by bioinformatics . the result showed that this protein was instable , p1 9 . 65 , located in bacterial inner membrane , and composed of alpha helix , extrended strand and random coil . there was a srong transmembrance domain and contained some conserved sites such as protein dinase c phosphorylation 通过互联网数据库及生物信息学分析工具进行初步分析表明:该基因编码的蛋白是一种等电点为9 . 65的不稳定蛋白,定位于细胞内膜;由螺旋、延伸带、和随机卷曲三种形式组成,具有蛋白激酶c磷酸化位点等多个位点,并具有一个强跨膜疏水区。
( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature . after 120d some cp cells died in the way of apoptosis . most nucleus of cells were condensed , and chromosome was marginated beside the nucleus inner membrane , cell sizes were reduced , dna ladder showed in dna gel electrophoresis ( 2 ) cp细胞系在10低温下细胞死亡率与时间成正比, 120d的细胞具典型的凋亡现象,即细胞核固缩、染色体边集在核膜内侧;细胞体积变小;琼脂糖凝胶电泳上显示特征性的“梯状”带。