Histidine a basic amino acid formed only rarely in proteins . it is formed from atp and 5 - phosphoribosyl pyrophosphate in a complex biosynthetic pathway . glutamate is an intermediate in its breakdown 组氨酸:是蛋白质中少有的一种碱性氨基酸。由atp和5 -磷酸核糖基焦磷酸盐通过复杂的生物合成途径合成。在其降解过程中可形成谷氨酸。
Structure analysis indicated that the molecule of hwtx - i consists of a small triple stranded anti - parallel ( 3 - sheet and five ( 3 - turns . the n - terminal , c - terminal and most basic amino acid residues are located in the surface of the molecule 构象研究表明, hwtx -由一小段三股反平行的-折叠和5个-转角组成,肽链的n端和c端以及多数碱性氨基酸残基都分布在分子表面。
6 . the ratio of acidic amino acids to basic amino acids is high in all of the spongy body , acid - sm and acid - ism . there are high contents of glycine ( gly ) and serine ( ser ) , which are usually related to the function of framework in the mineralization 6 、氨基酸分析表明酸性氨基酸含量较高,与钙的吸附有关, gly和ser在背盾和不溶性有机质中高,预示与文石形成相关的胶原和纤维蛋白物质含量高。
The sequence 56 analysis shows that there is a long open reading frame in it which encode a protein of 364 animo acid residues . the m protein include 47 basic amino acid , 30 acidic amino acid , which cause a basic estimated pi of 9 . 7 . phylogenetic tree based on ndv matrix protein gene shows that the b95 and v4 has the most close relationship than other ndv strain 关于ndvm蛋白国内未见有报道,国外对此蛋白的研究也十分有限,本文克隆出了ndv株b95m蛋白基因的全序列,为m蛋白的表达及进一步研究其在病毒复制与致病中的作用机理打下了基础。
The gene was 1668bp in length , encoding the f protein composed of 553 amino acids . sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids , six potential glycosylation sites and thirteen cysteines . a sequence region of basic amino acids , rrqrrf , was found at the f , - f2 cleavage site , indicating that f4ge9 was a typical virulent strain 序列分析和二级结构预测表明, f _ ( 48 ) e _ ( 9 )株f蛋白含有3个分别由25个氨基酸组成的疏水区,存在6个潜在的糖基化位点, 13个半胱氨酸残基,裂解位点区域的氨基酸序列为rrqrrf ,说明f _ ( 48 ) e _ 9株是一株典型的强毒株。