条件培养基的英文

例句与用法

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1. The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment . in brief , the intact blastocysts were plated on sto feeder layer treated with mitomycin , and were cultured in the media supplymented with brl condition medium
   联合evans和martin的方法，稍加改良来分离小鼠胚胎干细胞，把昆明白小鼠完整的囊胚直接种植在经丝裂霉素灭活的sto饲养层细胞上，在含有brl条件培养基的es细胞培养液中培养。
2. ( 7 ) in order to establish a feeeder - free system in porcine eg culture , we put pgcs on sto feeder layer , on sto extracellular matrix or on 0 . 1 % gelatin in brl - cm , and the results showed that when pgcs cultured on sto feeder , the brl - cm had a positive effect on keeping the undifferent state of eg colonies
   ( 7 )对非饲养层培养系统培养猪pgc进行了初步探索。实验结果证明，以sto为饲养层，培养液中加条件培养基对猪eg细胞保持不分化状态有积极作用，但培养板底铺有sto基质或明胶，即使用条件培养基也没有获得典型的eg集落。
3. These results are very important for us to further understand the genetic background , biological characteristics , evolutionary rule and the anti - schistosomajaponicum mechanism of microtus fortis at the molecular levels . the specific base changes of the dna fragme nt between the two subspecies are probably correlative with the animal immigration , survival conditions , and species evolution . the cdna library of microtus fortis bone marrow cells was transferred in situ to nylon membrane , which was divided into eight equals ( ga ~ - gh )
   利用已经建立的东方田鼠骨髓细胞质粒cdna文库，将cdna文库转化菌落印迹至尼龙膜，将膜均分成8份( ga gh ) ，制备基因池，分别培养、提取基因池质粒dna ，通过lipofect - 2000脂质体转染技术，将基因池质粒dna导入hek293细胞， 48h后收集转染细胞上清液，即条件培养基。
4. Based on the review of previous researches of es cells , we conducted to isolate mouse es cells from the icm of early blasteocyts , to proliferate es cell d3 line and to examine their ability of spontaneous differentiation in vitro , as well as to induce the es cell d3 line into osteoblasts by conditioned - medium , ascorbic acid , - glycerophosphate and / or dexame - thasone . 1
   本研究在回顾es细胞研究文献的基础上，进行了小鼠es细胞的分离与克隆、 es - d3细胞系的扩增和分化能力检测，以及应用成骨细胞条件培养基、维生素c 、 -磷酸甘油和地塞米松进行了定向诱导es - d3细胞系向成骨细胞的分化。