In addition , we are highly interested to study non - covalent interaction involved in the protein quaternary structure by using various biophysical methods ( including analytical ultracentrifugation and nanospray - esi - ms ) as these approaches can be applied to decipher protein - protein interaction 此外,我们也使用一些生物物理的方法,例如分析型超高速离心、电子喷?离子化质谱技术,探讨在蛋白质四级结构中的非共价性结合的交互作用,希望所得的结果能够应用在蛋白质与蛋白质的交互作用。
In escherichia coli , arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway . here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog , and the effects of n - terminus on feedback inhibition and its quaternary structure , and the importance of the structural " d2 " symmetry to allosteric inhibition 本博士论文工作以大肠杆菌k - 12来源的arog为研究对象,通过定点突变、反馈抑制实验和酶学动力学参数的测定,深入地研究了arog的反馈抑制位点的特性,并对arog的n -末端在反馈抑制机理和维持稳定四级结构中的作用,以及蛋白质结构的“ d2 ”对称性对酶功能的重要性等进行了具体的研究。
Besides , based on the block diagrams and layers design philosophy , the components and system reliability analysis cores , user interfaces and result display windows of the software were developed . the reliability parameters of the electronic system were predicted sequentially by components , cards , units and systems reliability analysis 同时,采用框图法和层次化结构设计思想编写了元器件可靠性分析模块及系统可靠性分析模块的程序内核、用户界面和结果显示界面,使本套软件按照元器件、板卡、部件和系统四级结构预测复杂电子设备的整体可靠性。